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Biochemistry Protein Purification Lab Techniques


CHASM: Charge, Hydrophobicity or solubility, Affinity, Stability, and Molecular weight or size.

C- charge – Ion Exchange Chromatography

Cation and Anion Exchange: charged beads get attached to the proteins of the right charge

H: Salting out: Hydrophobicity

When you add salt such as NaCl to a solution of protein and water, eventually protein will precipitate out. The more hydrophobic protein the less salt it needs to precipitate out. Why? Salt ions will interact with water freeing proteins and making them come out

A-affinity- Affinity Chromotography

The columns is packed with beads. To these beads we attach specific group for the protein we’re trying to catch.

S- stability, for example heat

M – molecular weight